Share Email Print
cover

Proceedings Paper

One-micron resolution optical coherence tomography (OCT) in vivo for cellular level imaging
Author(s): Dongyao Cui; Xinyu Liu; Jing Zhang; Xiaojun Yu; Ding Sun; Yuemei Luo; Jun Gu; Ping Shum; Linbo Liu
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

We developed a spectral domain OCT system combining two NIR, CW light sources of different spectral range. Its resolving power is validated by visualizing the cellular structures of zebra fish larvae in vivo. An NIR extended illumination from 755-1100 nm is achieved. The axial resolution is 1.27 μm in air, corresponding to 0.93μm in tissue (n=1.36), which is the highest axial resolution using NIR, CW laser sources up to date to the best of our knowledge. In vivo imaging is conducted to demonstrate the resolving power of proposed one-micron resolution OCT system. The top and bottom surfaces of individual disk-like red blood cell is reliably visualized, as well as flat, spindle shaped endothelial cells lining along the luminal surface of the blood vessel wall. This study provides a viable solution for cellular and subcellular level OCT imaging system which is also very competitive in cost.

Paper Details

Date Published: 2 March 2015
PDF: 6 pages
Proc. SPIE 9312, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX, 93123R (2 March 2015); doi: 10.1117/12.2076438
Show Author Affiliations
Dongyao Cui, Nanyang Technological Univ. (Singapore)
Nanyang Technological Univ., CINTRA, CNRS (Singapore)
Xinyu Liu, Nanyang Technological Univ. (Singapore)
Jing Zhang, Nanyang Technological Univ. (Singapore)
Xiaojun Yu, Nanyang Technological Univ. (Singapore)
Ding Sun, Nanyang Technological Univ. (Singapore)
Yuemei Luo, Nanyang Technological Univ. (Singapore)
Jun Gu, Nanyang Technological Univ. (Singapore)
Ping Shum, Nanyang Technological Univ. (Singapore)
Nanyang Technological Univ., CINTRA, CNRS (Singapore)
Linbo Liu, Nanyang Technological Univ. (Singapore)


Published in SPIE Proceedings Vol. 9312:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIX
James G. Fujimoto; Joseph A. Izatt; Valery V. Tuchin, Editor(s)

© SPIE. Terms of Use
Back to Top