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Proceedings Paper

Laser-induced perturbation into molecular dynamics localized in neuronal cell
Author(s): Chie Hosokawa; Naoko Takeda; Suguru N. Kudoh; Takahisa Taguchi
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Paper Abstract

Molecular dynamics at synaptic terminals in neuronal cells is essential for synaptic plasticity and subsequent modulation of cellular functions in a neuronal network. For realizing artificial control of living neuronal network, we demonstrate laser-induced perturbation into molecular dynamics in the neuronal cells. The optical trapping of cellular molecules such as synaptic vesicles or neural cell adhesion molecules labeled with quantum dots was evaluated by fluorescence imaging and fluorescence correlation spectroscopy. The trapping and assembling dynamics was revealed that the molecular motion was constrained at the focal spot of a focused laser beam due to optical trapping force. Our method has a potential to manipulate synaptic transmission at single synapse level.

Paper Details

Date Published: 10 March 2015
PDF: 5 pages
Proc. SPIE 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II, 93052N (10 March 2015); doi: 10.1117/12.2076348
Show Author Affiliations
Chie Hosokawa, National Institute of Advanced Industrial Science and Technology (Japan)
Naoko Takeda, National Institute of Advanced Industrial Science and Technology (Japan)
Kwansei Gakuin Univ. (Japan)
Suguru N. Kudoh, Kwansei Gakuin Univ. (Japan)
Takahisa Taguchi, National Institute of Advanced Industrial Science and Technology (Japan)
National Institute of Information and Communications Technology (Japan)


Published in SPIE Proceedings Vol. 9305:
Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II
Henry Hirschberg; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Steen J. Madsen, Editor(s)

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