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Proceedings Paper

Optimisation of acquisition time in bioluminescence imaging
Author(s): Shelley L. Taylor; Suzannah K. G. Mason; Sophie Glinton; Mark Cobbold; Iain B. Styles; Hamid Dehghani
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Paper Abstract

Decreasing the acquisition time in bioluminescence imaging (BLI) and bioluminescence tomography (BLT) will enable animals to be imaged within the window of stable emission of the bioluminescent source, a higher imaging throughput and minimisation of the time which an animal is anaesthetised. This work investigates, through simulation using a heterogeneous mouse model, two methods of decreasing acquisition time: 1. Imaging at fewer wavelengths (a reduction from five to three); and 2. Increasing the bandwidth of filters used for imaging. The results indicate that both methods are viable ways of decreasing the acquisition time without a loss in quantitative accuracy. Importantly, when choosing imaging wavelengths, the spectral attenuation of tissue and emission spectrum of the source must be considered, in order to choose wavelengths at which a high signal can be achieved. Additionally, when increasing the bandwidth of the filters used for imaging, the bandwidth must be accounted for in the reconstruction algorithm.

Paper Details

Date Published: 5 March 2015
PDF: 6 pages
Proc. SPIE 9319, Optical Tomography and Spectroscopy of Tissue XI, 93191T (5 March 2015); doi: 10.1117/12.2075661
Show Author Affiliations
Shelley L. Taylor, The Univ. of Birmingham (United Kingdom)
Suzannah K. G. Mason, The Univ. of Birmingham (United Kingdom)
Sophie Glinton, The Univ. of Birmingham (United Kingdom)
Mark Cobbold, The Univ. of Birmingham (United Kingdom)
Iain B. Styles, The Univ. of Birmingham (United Kingdom)
Hamid Dehghani, The Univ. of Birmingham (United Kingdom)


Published in SPIE Proceedings Vol. 9319:
Optical Tomography and Spectroscopy of Tissue XI
Bruce J. Tromberg; Arjun G. Yodh; Eva Marie Sevick-Muraca; Robert R. Alfano, Editor(s)

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