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Proceedings Paper

Efficient delivery of quantum dots in live cells by gold nanoparticle mediated photoporation
Author(s): Ranhua Xiong; Freya Joris; Ine De Cock; Jo Demeester; Stefaan C. De Smedt; Andre G. Skirtach; Kevin Braeckmans
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Paper Abstract

There is considerable interest in using Quantum Dots (QDs) as fluorescent probes such for cellular imaging due to unique advantages in comparison with conventional molecular dyes. However, cytosolic delivery of QDs into live cells remains a major challenge. Here we demonstrate highly efficient delivery of PEG-coated QDs into live cells by means of laser-induced vapour nanobubbles. Using this procedure we succeeded in high-throughput loading of ~80% of cells while maintaining a cell viability of ~85%.

Paper Details

Date Published: 12 March 2015
PDF: 5 pages
Proc. SPIE 9338, Colloidal Nanoparticles for Biomedical Applications X, 93380X (12 March 2015); doi: 10.1117/12.2075659
Show Author Affiliations
Ranhua Xiong, Ghent Univ. (Belgium)
Freya Joris, Ghent Univ. (Belgium)
Ine De Cock, Ghent Univ. (Belgium)
Jo Demeester, Ghent Univ. (Belgium)
Stefaan C. De Smedt, Ghent Univ. (Belgium)
Andre G. Skirtach, Ghent Univ. (Belgium)
Max-Planck Institute of Colloids and Interfaces (Germany)
Kevin Braeckmans, Ghent Univ. (Belgium)

Published in SPIE Proceedings Vol. 9338:
Colloidal Nanoparticles for Biomedical Applications X
Wolfgang J. Parak; Marek Osinski; Xing-Jie Liang, Editor(s)

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