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Proceedings Paper

Optical clearing of the mouse brain and light attenuation quantitation
Author(s): Angela d'Esposito; Daniil Nikitichev; Adrien Desjardins; Simon Walker-Samuel; Mark F. Lythgoe
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Paper Abstract

Optical clearing allows the reduction of light scattering in biological tissue, enabling 3D morphological information to be obtained deep within tissue using techniques such as optical projection tomography and light sheet microscopy. However, the extent of the clearing is dependent on the technique that is used. There is therefore a need for methods to quantify the quality of the clearing process and thereby to compare clearing techniques. In this study, we developed such a method using a custom spectroscopy system and applied it to compare three techniques that were applied to mouse brain: BABB (Murray’s clear), pBABB (a modification of BABB which includes the use of hydrogen peroxide), and passive CLARITY.

Paper Details

Date Published: 13 March 2015
PDF: 7 pages
Proc. SPIE 9321, Optical Interactions with Tissue and Cells XXVI, 93210R (13 March 2015); doi: 10.1117/12.2075580
Show Author Affiliations
Angela d'Esposito, Univ. College London (United Kingdom)
Daniil Nikitichev, Univ. College London (United Kingdom)
Adrien Desjardins, Univ. College London (United Kingdom)
Simon Walker-Samuel, Univ. College London (United Kingdom)
Mark F. Lythgoe, Univ. College London (United Kingdom)

Published in SPIE Proceedings Vol. 9321:
Optical Interactions with Tissue and Cells XXVI
E. Duco Jansen, Editor(s)

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