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Proceedings Paper

Fluorescence lifetime imaging of deep-seated fluorophore in turbid medium
Author(s): Alexander V. Khilov; I. I. Fiks; Vladimir I. Plehanov; M. Yu. Kirillin; I. V. Turchin
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Paper Abstract

Correct identification of different fluorophores in the fluorescence lifetime imaging in vivo requires accounting for distortion of the measured fluorescent kinetics curve due to light scattering and absorption in medium. This distortion induces the difference between real and measured lifetimes of a fluorophore. We obtained analytical expression based on diffuse approximation of radiation transfer equation that allows to refine estimating the lifetime of a fluorophore. It was shown that our approach can be applied both for analytic kinetics curves obtained by diffuse approximation, Monte Carlo simulated curves and results of model experiment. Analytical and Monte Carlo simulated curves were obtained for media with different optical properties and lifetimes corresponding to those of real fluorophores. Results of numerical simulation are confirmed by the results of the model experiment.

Paper Details

Date Published: 5 March 2015
PDF: 9 pages
Proc. SPIE 9319, Optical Tomography and Spectroscopy of Tissue XI, 93190S (5 March 2015); doi: 10.1117/12.2075522
Show Author Affiliations
Alexander V. Khilov, Institute of Applied Physics (Russian Federation)
I. I. Fiks, Institute of Applied Physics of the Russian Academy of Science (Russian Federation)
Vladimir I. Plehanov, Institute of Applied Physics (Russian Federation)
M. Yu. Kirillin, Institute of Applied Physics of the Russian Academy of Science (Russian Federation)
I. V. Turchin, Institute of Applied Physics of the Russian Academy of Science (Russian Federation)


Published in SPIE Proceedings Vol. 9319:
Optical Tomography and Spectroscopy of Tissue XI
Bruce J. Tromberg; Arjun G. Yodh; Eva Marie Sevick-Muraca; Robert R. Alfano, Editor(s)

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