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Proceedings Paper

Tissue healing response following hyperthermic vapor ablation in the porcine longissimus muscle
Author(s): John T. Grantham; Brian T. Grisez; Justin Famoso; Michael Hoey; Chris Dixon; James E. Coad
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Paper Abstract

As the use of hyperthermic ablation technologies has increased, so too has the need to understand their effects on tissue and their healing responses. This study was designed to characterize tissue injury and healing following hyperthermic vapor ablation in the in vivo porcine longissimus muscle model. The individual ablations were performed using the NxThera Vapor Delivery System (NxThera Inc., Minneapolis, MN). To assess the vapor ablation’s evolution, the swine were euthanized post-treatment on Day 0, Day 3, Day 7, Day 14, Day 28, Day 45 and Day 90. Triphenyltetrazolium chloride viability staining (TTC staining) was used to macroscopically assess the extent of each vapor ablation within the tissue. The ablation associated healing responses were then histologically evaluated for acute inflammation, chronic inflammation, foreign body reaction and fibrosis. Two zones of tissue injury were initially identified in the ablations: 1) a central zone of complete coagulative necrosis and 2) an outer “transition zone” of viable and non-viable cells. The ablations initially increased in size from Day 0 to Day 7 and then progressively decreased in size though Day 45. The initial Day 3 healing changes originated in the transition zone with minimal acute and chronic inflammation. As time progressed, granulation tissue began to form by Day 7 and peaked around Day 14. Collagen formation, deposition and remodeling began in the adjacent healthy tissue by Day 28, replaced the ablation site by Day 45 and reorganized by Day 90. In conclusion, this vapor ablation technology provided a non-desiccating form of hyperthermic ablation that resulted in coagulative necrosis without a central thermally/heat-fixed tissue component, followed a classical wound healing pathway, and healed with minimal associated inflammation.

Paper Details

Date Published: 11 March 2015
PDF: 8 pages
Proc. SPIE 9326, Energy-based Treatment of Tissue and Assessment VIII, 93260E (11 March 2015); doi: 10.1117/12.2075410
Show Author Affiliations
John T. Grantham, West Virginia Univ. (United States)
Brian T. Grisez, West Virginia Univ. (United States)
Justin Famoso, West Virginia Univ. (United States)
Michael Hoey, NxThera, Inc. (United States)
Chris Dixon, New York Medical College (United States)
James E. Coad, West Virginia Univ. (United States)


Published in SPIE Proceedings Vol. 9326:
Energy-based Treatment of Tissue and Assessment VIII
Thomas P. Ryan, Editor(s)

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