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Proceedings Paper

WP1: transgenic opto-animals
Author(s): E. Użarowska; Rafał Czajkowski; W. Konopka
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Paper Abstract

We aim to create a set of genetic tools where permanent opsin expression (ChR or NpHR) is precisely limited to the population of neurons that express immediate early gene c-fos during a specific temporal window of behavioral training. Since the c-fos gene is only expressed in neurons that form experience-dependent ensemble, this approach will result in specific labeling of a small subset of cells that create memory trace for the learned behavior. To this end we employ two alternative inducible gene expression systems: Tet Expression System and Cre/lox System. In both cases, the temporal window for opsin induction is controlled pharmacologically, by doxycycline or tamoxifen, respectively. Both systems will be used for creating lines of transgenic animals.

Paper Details

Date Published: 25 November 2014
PDF: 9 pages
Proc. SPIE 9290, Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2014, 92901V (25 November 2014); doi: 10.1117/12.2075210
Show Author Affiliations
E. Użarowska, Nencki Institute of Experimental Biology (Poland)
Rafał Czajkowski, Nencki Institute of Experimental Biology (Poland)
W. Konopka, Nencki Institute of Experimental Biology (Poland)


Published in SPIE Proceedings Vol. 9290:
Photonics Applications in Astronomy, Communications, Industry, and High-Energy Physics Experiments 2014
Ryszard S. Romaniuk, Editor(s)

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