Share Email Print
cover

Proceedings Paper

Strategies for leukemic biomarker detection using long-range surface plasmon-polaritons
Author(s): O. Krupin; C. Wang; P. Berini
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

The suitability and use of long-range surface plasmon-polaritons for leukemic biomarker detection is discussed. A novel optical biosensor comprised of gold straight waveguides embedded in CYTOP with an etched microfluidic channel was tested for detecting leukemia in patient serum. Gold surface functionalization involved the interaction of protein G (PG) with antibodies by first adsorbing PG on bare gold and then antibodies (Immunoglobulin G, IgG). Differentiation between healthy and leukemia patients was based on the difference in ratios of Ig kappa (Igκ) and Ig lambda (Igλ) light chains in both serums. The ratio for a normal patient is ~1.4 - 2, whereas for a leukemia patient this ratio is altered. As a receptor (primary antibodies), goat anti-human anti-IgGκ and anti-IgGλ were used to functionalize the surface. Diluted normal and leukemia patient serums were tested over the aforementioned surfaces. The ratios of mass surface densities of IgGκ:IgGλ for normal serum (NS) and patient serum (PS) were found to be 1.55 and 1.92 respectively.

Paper Details

Date Published: 25 September 2014
PDF: 11 pages
Proc. SPIE 9288, Photonics North 2014, 92880W (25 September 2014); doi: 10.1117/12.2075204
Show Author Affiliations
O. Krupin, Univ. of Ottawa (Canada)
C. Wang, Mount Sinai Hospital, Univ. of Toronto (Canada)
P. Berini, Univ. of Ottawa (Canada)


Published in SPIE Proceedings Vol. 9288:
Photonics North 2014
Steve MacLean; David V. Plant, Editor(s)

© SPIE. Terms of Use
Back to Top