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Proceedings Paper

Time-resolved fluorescence monitoring of cholesterol in peripheral blood mononuclear cells
Author(s): Z. Martinakova; J. Horilova; I. Lajdova; A. Marcek Chorvatova
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Paper Abstract

Precise evaluation of intracellular cholesterol distribution is crucial for improving diagnostics of diseased states associated with cholesterol alteration. Time-resolved fluorescence techniques are tested for non-invasive investigation of cholesterol in living cells. Fluorescent probe NBD attached to cholesterol was employed to evaluate cholesterol distribution in peripheral blood mononuclear cells (PBMC) isolated from the human blood. Fluorescence Lifetime Imaging Microscopy (FLIM) was successfully applied to simultaneously monitor the spatial distribution and the timeresolved characteristics of the NBD-cholesterol fluorescence in PBMC. Gathered data are the first step in the development of a new perspective non-invasive diagnostic method for evaluation of cholesterol modifications in diseases associated with disorders of lipid metabolism.

Paper Details

Date Published: 5 December 2014
PDF: 9 pages
Proc. SPIE 9441, 19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics, 94411L (5 December 2014); doi: 10.1117/12.2074288
Show Author Affiliations
Z. Martinakova, International Laser Ctr. (Slovakia)
Trnava Univ. (Slovakia)
J. Horilova, International Laser Ctr. (Slovakia)
Pavol Jozef Safarik Univ. (Slovakia)
I. Lajdova, Slovak Medical Univ. (Slovakia)
A. Marcek Chorvatova, International Laser Ctr. (Slovakia)
Univ. of Cyril and Methodius (Slovakia)


Published in SPIE Proceedings Vol. 9441:
19th Polish-Slovak-Czech Optical Conference on Wave and Quantum Aspects of Contemporary Optics
Agnieszka Popiolek-Masajada; Waclaw Urbanczyk, Editor(s)

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