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Proceedings Paper

Digital image analysis of striated skeletal muscle tissue injury during reperfusion after induced ischemia
Author(s): Doris Haydee Rosero Salazar; Liliana Salazar Monsalve
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Paper Abstract

Conditions such as surgical procedures or vascular diseases produce arterial ischemia and reperfusion injuries, which generate changes in peripheral tissues and organs, for instance, in striated skeletal muscle. To determine such changes, we conducted an experimental method in which 42 male Wistar rat were selected, to be undergone to tourniquet application on the right forelimb and left hind limb, to induce ischemia during one and three hours, followed by reperfusion periods starting at one hour and it was prolonged up to 32 days. Extensor carpi radialis longus and soleus respectively, were obtained to be processed for histochemical and morphometric analysis. By means of image processing and detection of regions of interest, variations of areas occupied by muscle fibers and intramuscular extracellular matrix (IM-ECM) throughout reperfusion were observed. In extensor carpi radialis longus, results shown reduction in the area occupied by muscle fibers; this change is significant between one hour and three hours ischemia followed by 16 hours, 48 hours and 32 days reperfusión (p˂0.005). To compare only periods of reperfusión that continued to three hours ischemia, were found significant differences, as well. For area occupied by IM-ECM, were identified increments in extensor carpi radialis longus by three hours ischemia and eight to 16 days reperfusion; in soleus, was observed difference by one hour ischemia with 42 hours reperfusion, and three hours ischemia followed by four days reperfusion (p˂0.005). Skeletal muscle develops adaptive changes in longer reperfusion, to deal with induced injury. Descriptions beyond 32 days reperfusion, can determine recovering normal pattern.

Paper Details

Date Published: 28 January 2015
PDF: 9 pages
Proc. SPIE 9287, 10th International Symposium on Medical Information Processing and Analysis, 92870I (28 January 2015); doi: 10.1117/12.2073825
Show Author Affiliations
Doris Haydee Rosero Salazar, Univ. del Valle (Colombia)
Liliana Salazar Monsalve, Univ. del Valle (Colombia)

Published in SPIE Proceedings Vol. 9287:
10th International Symposium on Medical Information Processing and Analysis
Eduardo Romero; Natasha Lepore, Editor(s)

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