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Proceedings Paper

Ex-vivo multi-modal microscopy of healthy skin
Author(s): Edgar Guevara; José Manuel Gutiérrez-Hernández; Alexandre Castonguay; Frédéric Lesage; Francisco Javier González
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Paper Abstract

The thorough characterization of skin samples is a critical step in investigating dermatological diseases. The combination of depth-sensitive anatomical imaging with molecular imaging has the potential to provide vast information about the skin. In this proof-of-concept work we present high-resolution mosaic images of skin biopsies using Optical Coherence Tomography (OCT) manually co-registered with standard microscopy, bi-dimensional Raman spectral mapping and fluorescence imaging. A human breast skin sample, embedded in paraffin, was imaged with a swept-source OCT system at 1310 nm. Individual OCT volumes were acquired in fully automated fashion in order to obtain a large field-of-view at high resolution (~10μm). Based on anatomical features, the other three modalities were manually co-registered to the projected OCT volume, using an affine transformation. A drawback is the manual co-registration, which may limit the utility of this method. However, the results indicate that multiple imaging modalities provide complementary information about the sample. This pilot study suggests that multi-modal microscopy may be a valuable tool in the characterization of skin biopsies.

Paper Details

Date Published: 25 September 2014
PDF: 6 pages
Proc. SPIE 9288, Photonics North 2014, 92880P (25 September 2014); doi: 10.1117/12.2073702
Show Author Affiliations
Edgar Guevara, Ecole Polytechnique de Montréal (Canada)
José Manuel Gutiérrez-Hernández, Univ. Autónoma de San Luis Potosí (Mexico)
Alexandre Castonguay, Ecole Polytechnique de Montréal (Canada)
Montreal Heart Institute (Canada)
Frédéric Lesage, Ecole Polytechnique de Montréal (Canada)
Montreal Heart Institute (Canada)
Francisco Javier González, Univ. Autónoma de San Luís Potosí (Mexico)

Published in SPIE Proceedings Vol. 9288:
Photonics North 2014
Steve MacLean; David V. Plant, Editor(s)

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