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Proceedings Paper

Phase-contrast scanning optical microscopy for biological tissues
Author(s): Masaki Hisaka
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Paper Abstract

Phase-contrast imaging of living tissues is important for investigating biological structures and functions. The phasecontrast scanning optical microscope using annular illumination and image processing has been developed as a new tool for visualizing phase objects. This microscope has excellent features, such as separate phase and amplitude imaging, reducing multiscattering effects and spherical aberration-free imaging. Using a figure-8-shaped spatial-frequency filter with sign correction in order to extract linear phase-contrast components, we observed the phase-contrast structures of biological tissue samples and evaluated the microscope’s imaging features.

Paper Details

Date Published: 5 November 2014
PDF: 6 pages
Proc. SPIE 9272, Optical Design and Testing VI, 92720B (5 November 2014); doi: 10.1117/12.2072566
Show Author Affiliations
Masaki Hisaka, Osaka Electro-Communication Univ. (Japan)


Published in SPIE Proceedings Vol. 9272:
Optical Design and Testing VI
Yongtian Wang; Chunlei Du; José Sasián; Kimio Tatsuno, Editor(s)

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