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Proceedings Paper

Quantitative changes of collagen in human normal breast tissue and invasive ductal carcinoma using nonlinear optical microscopy
Author(s): Weiqiang Li; Yan Wu; Yuane Lian; Fangmeng Fu; Chuan Wang; Shuangmu Zhuo; Jianxin Chen
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Paper Abstract

Multiphoton microscopy (MPM) imaging of collagen plays a key role in noninvasive diagnosis of human tissue. During the experiment, we observed an interesting phenomenon which two-photon excited fluorescence (TPEF) signal of collagen in human invasive ductal carcinoma of breast tissue becomes much weaker than the normal breast tissue, but the second harmonic generation (SHG) signal of collagen does not get an obvious change . In order to explain the phenomena,this paper emphasizes on the intensity of TPEF and SHG signal from collagen in human invasive ductal carcinoma of breast tissues and normal breast tissue. Further, we respectively obtain the intensity spectral information from collagen in the above two tissues with all parameter unaltered. Our quantitative results show that the intensity of TPEF from collagen in human invasive ductal carcinoma of breast tissue is much lower than the intensity of TPEF from collagen in normal breast tissue. According to the theoretic analysis, it was concluded that the intensity of TPEF declined due to the reduction of the quantum yield when the collagen was intruded by cancer cells. However, the invasion of cancer cells has no effect on decisive factor of SHG. Our theoretical analysis brings more detailed information about intensity of SHG and TPEF from collagen in the above two tissues.

Paper Details

Date Published: 20 November 2014
PDF: 8 pages
Proc. SPIE 9268, Optics in Health Care and Biomedical Optics VI, 926827 (20 November 2014); doi: 10.1117/12.2071685
Show Author Affiliations
Weiqiang Li, Fujian Normal Univ. (China)
Yan Wu, Fujian Normal Univ. (China)
Jimei Univ. (China)
Yuane Lian, The Affiliated Union Hospital, Fujian Medical Univ. (China)
Fangmeng Fu, The Affiliated Union Hospital, Fujian Medical Univ. (China)
Chuan Wang, The Affiliated Union Hospital, Fujian Medical Univ. (China)
Shuangmu Zhuo, Fujian Normal Univ. (China)
Jianxin Chen, Fujian Normal Univ. (China)


Published in SPIE Proceedings Vol. 9268:
Optics in Health Care and Biomedical Optics VI
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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