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Proceedings Paper

Role of laser fluence in protein synthesis of cultured DRG neurons following low-level laser irradiation
Author(s): Liqin Zheng; Caimin Qiu; Yuhua Wang; Yixiu Zeng; Hongqin Yang; Yanding Zhang; Shusen Xie
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Paper Abstract

Low-level lasers have been used to relieve pain in clinical for many years. But the mechanism is not fully clear. In animal models, nitric oxide (NO) has been reported involving in the transmission and modulation of nociceptive signals. So the objective of this study was to establish whether low-level laser with different fluence could stimulate the production of nitric oxide synthese (NOS), which produces NO in cultured primary dorsal root ganglion neurons (DRG neurons). The primary DRG neurons were isolated from healthy Sprague Dawley rats (8-12 weeks of age) and spread on 35 mm culture dishes specially used for confocal microscopy. 24 hours after spreading, cells were irradiated with 658 nm laser for two consecutive days at the energy density of 20, 40, 60 and 80 mJ·cm-2 respectively. Control groups were not exposed to the laser, but were kept under the same conditions as the irradiated ones. The synthesis of NOS after laser irradiation was detected by immunofluorescence assay, and the changes of NOS were evaluated using confocal microscopy and Image J software. The results showed that all the laser fluence could promote the production of NOS in DRG neurons, especially the 60 mJ·cm-2 . These results demonstrated that low-level laser irradiation could modify protein synthesis in a dose- or fluence- dependent manner, and indicated that low-level laser irradiation might achieve the analgesic effect through modulation of NO production.

Paper Details

Date Published: 18 November 2014
PDF: 6 pages
Proc. SPIE 9268, Optics in Health Care and Biomedical Optics VI, 92680I (18 November 2014); doi: 10.1117/12.2071447
Show Author Affiliations
Liqin Zheng, Fujian Normal Univ. (China)
Caimin Qiu, Fujian Normal Univ. (China)
Yuhua Wang, Fujian Normal Univ. (China)
Yixiu Zeng, Fujian Normal Univ. (China)
Hongqin Yang, Fujian Normal Univ. (China)
Yanding Zhang, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 9268:
Optics in Health Care and Biomedical Optics VI
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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