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Proceedings Paper

Identification of the boundary between normal breast tissue and invasive ductal carcinoma during breast-conserving surgery using multiphoton microscopy
Author(s): Tongxin Deng; Yuting Nie; Yuane Lian; Yan Wu; Fangmeng Fu; Chuan Wang; Shuangmu Zhuo; Jianxin Chen
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Paper Abstract

Breast-conserving surgery has become an important way of surgical treatment for breast cancer worldwide nowadays. Multiphoton microscopy (MPM) has the ability to noninvasively visualize tissue architectures at the cellular level using intrinsic fluorescent molecules in biological tissues without the need for fluorescent dye. In this study, MPM is used to image the microstructures of terminal duct lobular unit (TDLU), invasive ductal carcinoma and the boundary region between normal and cancerous breast tissues. Our study demonstrates that MPM has the ability to not only reveal the morphological changes of the cuboidal epithelium, basement membrane and interlobular stroma but also identify the boundary between normal breast tissue and invasive ductal carcinoma, which correspond well to the Hematoxylin and Eosin (H and E) images. Predictably, MPM can monitor surgical margins in real time and provide considerable accuracy for resection of breast cancerous tissues intraoperatively. With the development of miniature, real-time MPM imaging technology, MPM should have great application prospects during breast-conserving surgery.

Paper Details

Date Published: 18 November 2014
PDF: 7 pages
Proc. SPIE 9268, Optics in Health Care and Biomedical Optics VI, 92681A (18 November 2014); doi: 10.1117/12.2070795
Show Author Affiliations
Tongxin Deng, Fujian Normal Univ. (China)
Yuting Nie, Fujian Normal Univ. (China)
Yuane Lian, The Affiliated Union Hospital, Fujian Medical Univ. (China)
Yan Wu, Fujian Normal Univ. (China)
Jimei Univ. (China)
Fangmeng Fu, The Affiliated Union Hospital, Fujian Medical Univ. (China)
Chuan Wang, The Affiliated Union Hospital, Fujian Medical Univ. (China)
Shuangmu Zhuo, Fujian Normal Univ. (China)
Jianxin Chen, Fujian Normal Univ. (China)


Published in SPIE Proceedings Vol. 9268:
Optics in Health Care and Biomedical Optics VI
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang, Editor(s)

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