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Proceedings Paper

Chemical modulation of the ultra-weak photon emission from Saccharomyces cerevisiae and differentiated HL-60 cells
Author(s): Kateřina Červinková; Michaela Nerudová; Jiří Hašek; Michal Cifra
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Paper Abstract

The ultra-weak photon emission (UPE) is a universal phenomenon common to all cells with active oxidative metabolism. Generally accepted mechanism of the origin of the ultra-weak photon emission considers reactions of radical or nonradical reactive oxygen species (ROS) with biomolecules such as lipids and proteins which lead to the formation of electron excited species. During the transition to the ground state the excess energy is released as a photon with a wavelength in the visible range of the electromagnetic spectrum. Since the intensity of the light is very low it is possible to be measured only by highly sensitive devices. We used Hamamatsu Photonics PMT module H7360-01 mounted into a light-tight chamber for the purposes of this work. The goal of our research is to delineate an origin of UPE from two model organisms; differentiated HL-60 cells (human promyelocytic leukemia) and yeast cells Saccharomyces cerevisiae. While the UPE from the yeast cells arises spontaneously during the growth without any external stimuli, UPE from HL-60 is induced by phorbol 12-myristate, 13-acetate (PMA). It is possible to modulate the UPE production by certain antioxidants which scavenge ROS formed during the metabolism (yeast cells) or respiratory burst (HL-60 cells). The experiments are focused on the description of effects caused by antioxidants. Several kinds of antioxidants (ascorbic acid, mannitol, glutathione) with different concentration were used and we studied the changes in the UPE intensities of and the temporal developments of the optical signal.

Paper Details

Date Published: 6 January 2015
PDF: 7 pages
Proc. SPIE 9450, Photonics, Devices, and Systems VI, 94500P (6 January 2015); doi: 10.1117/12.2070424
Show Author Affiliations
Kateřina Červinková, Czech Technical Univ. in Prague (Czech Republic)
Institute of Photonics and Electronics of the ASCR, v.v.i. (Czech Republic)
Michaela Nerudová, Czech Technical Univ. in Prague (Czech Republic)
Institute of Photonics and Electronics of the ASCR, v.v.i. (Czech Republic)
Jiří Hašek, Institute of Microbiology of the ASCR, v.v.i. (Czech Republic)
Michal Cifra, Institute of Photonics and Electronics of the ASCR, v.v.i. (Czech Republic)


Published in SPIE Proceedings Vol. 9450:
Photonics, Devices, and Systems VI
Pavel Tománek; Dagmar Senderáková; Petr Páta, Editor(s)

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