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Proceedings Paper

Concentration determination of collagen and proteoglycan in bovine nasal cartilage by Fourier transform infrared imaging and PLS
Author(s): Xuexi Zhang; Zhi-Yan Xiao; Jianhua Yin; Yang Xia
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Paper Abstract

Fourier transform infrared imaging (FTIRI) combined with chemometrics can be used to detect the structure of bio-macromolecule, measure the concentrations of some components, and so on. In this study, FTIRI with Partial Least-Squares (PLS) regression was applied to study the concentration of two main components in bovine nasal cartilage (BNC), collagen and proteoglycan. An infrared spectrum library was built by mixing the collagen and chondroitin 6-sulfate (main of proteoglycan) at different ratios. Some pretreatments are needed for building PLS model. FTIR images were collected from BNC sections at 6.25μm and 25μm pixel size. The spectra extracted from BNC-FTIR images were imported into the PLS regression program to predict the concentrations of collagen and proteoglycan. These PLS-determined concentrations are agreed with the result in our previous work and biochemical analytical results. The prediction shows that the concentrations of collagen and proteoglycan in BNC are comparative on the whole. However, the concentration of proteoglycan is a litter higher than that of collagen, to some extent.

Paper Details

Date Published: 17 September 2014
PDF: 7 pages
Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 923015 (17 September 2014); doi: 10.1117/12.2070165
Show Author Affiliations
Xuexi Zhang, Nanjing Univ. of Aeronautics and Astronautics (China)
Zhi-Yan Xiao, Washington Univ. in St. Louis (United States)
Jianhua Yin, Nanjing Univ. of Aeronautics and Astronautics (China)
Yang Xia, Oakland Univ. (United States)


Published in SPIE Proceedings Vol. 9230:
Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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