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Proceedings Paper

Optical biosensor with poly[N-nonyl-3,6-bis(ethylenedioxythiophene)carbazole] matrix for monitoring of phenol derivatives
Author(s): Agnieszka Jędrychowska; Karol Malecha; Joanna Cabaj; Jadwiga Sołoducho
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Paper Abstract

The aim of the research was to develop an enzymatic, optical biosensor which provides quick and convenient determination of phenolic compounds in aqueous solutions. The biosensing strategy concerns design, fabrication and testing of a miniature ceramic-based biosensor which is destined for in-situ substrate monitoring. The base of the measuring system was fabricated using low temperature co-fired ceramics (LTCC) technology. The biocatalyst – laccase– was immobilized on the thin film of poly[N-nonyl-3,6-bis(ethylenedioxythiophene)carbazole] which provided good binding of the enzyme to the substrate and positively affected on the catalytic activity of the protein. In order to evaluate properties of the designed biosensor, its response for various concentrations of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diamonnium sal (ABTS) was measured. The optical biosensor produced by presented method could find applications in many fields, i.e. for detection of phenolic compounds in food products and beverages, in industry for control of technological processes or for environmental monitoring

Paper Details

Date Published: 19 August 2014
PDF: 5 pages
Proc. SPIE 9291, 13th International Scientific Conference on Optical Sensors and Electronic Sensors, 929105 (19 August 2014); doi: 10.1117/12.2069987
Show Author Affiliations
Agnieszka Jędrychowska, Wrocław Univ. of Technology (Poland)
Karol Malecha, Wrocław Univ. of Technology (Poland)
Joanna Cabaj, Wrocław Univ. of Technology (Poland)
Jadwiga Sołoducho, Wrocław Univ. of Technology (Poland)

Published in SPIE Proceedings Vol. 9291:
13th International Scientific Conference on Optical Sensors and Electronic Sensors
Jacek Golebiowski; Roman Gozdur, Editor(s)

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