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Proceedings Paper

Based on surface-enhanced Raman spectroscopy analysis of serum albumin in different stages of liver disease for early screening primary liver cancer
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Paper Abstract

Despite the introduction of high-technology methods of detection and diagnosis, screening of primary liver cancer (PLC) remains imperfect. To diagnosis PLC earlier, Surface-enhanced Raman spectroscopy (SERS) coupled with cellulose-acetate membrane electrophoresis were introduced to separate human serum albumin and SERS spectra. Three groups (15 normal persons’ samples, 17 hepatitis/cirrhosis samples, 15 cases of PLC) of serum albumin were tested. Silver colloid was used to obtain SERS spectra of human serum albumin. Principal component analysis (PCA) and linear discriminant analysis (LDA) were also employed for statistical analysis. The mean Raman spectra of three groups and the difference spectra of any two suggested that the albumin has changed in liver patients. Compared to normal groups, some Raman peaks have shifted or even disappeared in hepatitis/cirrhosis and PLCs groups. The sensitivity and specificity between PLCs and normal groups is 80% and 93.3%. Among hepatitis/cirrhosis and normal groups, the sensitivity is 88.2% and specificity is also 93.3%. Besides, the sensitivity and specificity between PLCs and hepatitis/cirrhosis groups is 86.7% and 76.5%. All the above data and results indicated that early screening of PLC is potential by SERS in different stages of liver disease before cancer occurs.

Paper Details

Date Published: 17 September 2014
PDF: 6 pages
Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 923010 (17 September 2014); doi: 10.1117/12.2069157
Show Author Affiliations
Fadian Liao, Fujian Normal Univ. (China)
Qiuyong Ruan, Fujian Normal Univ. (China)
Juqiang Lin, Fujian Normal Univ. (China)
Jinyong Lin, Fujian Normal Univ. (China)
Yongyi Zeng, The First Affiliated Hospital of Fujian Medical Univ. (China)
Ling Li, The First Affiliated Hospital of Fujian Medical Univ. (China)
Zufang Huang, Fujian Normal Univ. (China)
Nenrong Liu, Fujian Normal Univ. (China)
Rong Chen, Fujian Normal Univ. (China)


Published in SPIE Proceedings Vol. 9230:
Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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