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Proceedings Paper

Effects of cholesterol on plasma membrane lipid order in MCF-7 cells by two-photon microscopy
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Paper Abstract

Lipid rafts are cholesterol- and glycosphingolipids- enriched microdomains on plasma membrane surface of mammal cells, involved in a variety of cellular processes. Depleting cholesterol from the plasma membrane by drugs influences the trafficking of lipid raft markers. Optical imaging techniques are powerful tools to study lipid rafts in live cells due to its noninvasive feature. In this study, breast cancer cells MCF-7 were treated with different concentrations of MβCD to deplete cholesterol and an environmentally sensitive fluorescence probe, Laurdan was loaded to image lipid order by two-photon microscopy. The generalized polarization (GP) values were calculated to distinguish the lipid order and disorder phase. GP images and GP distributions of native and cholesterol-depleted MCF-7 cells were obtained. Our results suggest that even at low concentration (0.5 mM) of MβCD, the morphology of the MCF-7 cells changes. Small high GP areas (lipid order phase) decrease more rapidly than low GP areas (lipid disorder phase), indicating that lipid raft structure was altered more severely than nonraft domains. The data demonstrates that cholesterol dramatically affect raft coverage and plasma membrane fluidity in living cells.

Paper Details

Date Published: 17 September 2014
PDF: 6 pages
Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92300V (17 September 2014); doi: 10.1117/12.2069100
Show Author Affiliations
Yixiu Zeng, Fujian Normal Univ. (China)
Jianling Chen, Fujian Normal Univ. (China)
Hongqin Yang, Fujian Normal Univ. (China)
Yuhua Wang, Fujian Normal Univ. (China)
Hui Li, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 9230:
Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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