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Proceedings Paper

A dual-excitation approach for dynamic fluorescence molecular tomography
Author(s): Simin Zuo; Xu Cao; Fei Liu; Jing Bai; Jianwen Luo
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Paper Abstract

Fluorescence molecular tomography (FMT) can quantify fluorophore concentration based on the static assumption that the fluorophore concentration is time invariant during the multi-projection acquisition process. However, when investigating the kinetic behaviors of fluorophores, especially when the change of fluorophore concentration is fast, the static assumption would provide inadequate temporal information and bring noise in the reconstruction results. In this study, we propose a dual-excitation approach for dynamic FMT to effectively image the time-varying fluorophores. Dual-excitation with the same source-detector pair is employed to collect two fluorescence measurements with a fixed time interval. Then, linear extrapolation is applied to the two measurements to compensate the measurement data from the adjacent source-detector pair. With the compensated measurement data over 360° projections, a sequence of fluorophore concentration images can be reconstructed with Tikhonov regularization. Numerical simulation results demonstrate the feasibility of the proposed approach in improving the temporal resolution and reducing the noise in dynamic FMT.

Paper Details

Date Published: 17 September 2014
PDF: 7 pages
Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92300Q (17 September 2014); doi: 10.1117/12.2069003
Show Author Affiliations
Simin Zuo, Tsinghua Univ. (China)
Xu Cao, Xidian Univ. (China)
Fei Liu, Tsinghua Univ. (China)
Tsinghua-Peking Ctr. for Life Science (China)
Jing Bai, Tsinghua Univ. (China)
Jianwen Luo, Tsinghua Univ. (China)

Published in SPIE Proceedings Vol. 9230:
Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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