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Proceedings Paper

In vivo vascular imaging with adaptive optics confocal scanning fluorescence microscopy
Author(s): Zhibin Wang; Lin Wei; Yi He; Xiqi Li; Guohua Shi; Yudong Zhang
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Paper Abstract

Adaptive optics is implemented in a confocal scanning fluorescence microscope with wavefront sensorless scheme. Using the image sharpness as the optimization metric, aberration correction is performed to compensate both system- and specimen-induced aberrations by using stochastic parallel gradient descent algorithm based upon Zernike polynomial modes. In vivo vascular imaging of mice ear is completed and the results revealed the improved signal and resolution leading to in substantially enhanced image contrast with aberration correction which allowed us to detect clearer vasculature structures.

Paper Details

Date Published: 17 September 2014
PDF: 5 pages
Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 92301W (17 September 2014); doi: 10.1117/12.2068823
Show Author Affiliations
Zhibin Wang, Institute of Optics and Electronics (China)
Univ. of Chinese Academy of Sciences (China)
Lin Wei, Institute of Optics and Electronics (China)
Yi He, Institute of Optics and Electronics (China)
Xiqi Li, Institute of Optics and Electronics (China)
Guohua Shi, Institute of Optics and Electronics (China)
Yudong Zhang, Institute of Optics and Electronics (China)


Published in SPIE Proceedings Vol. 9230:
Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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