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Proceedings Paper

Visual detection of Akt mRNA in living cell using gold nanoparticle beacon
Author(s): Yi Ma; Caiping Tian; Siwen Li; Zhaohui Wang; Yueqing Gu
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Paper Abstract

PI3K-Akt signaling pathway plays the key role in cell apoptosis and survival, and the components of PI3K /Akt signaling pathway are often abnormally expressed in human tumors. Therefore, determination of the Akt (protein kinase B, PKB) messenger ribonucleic acid (mRNA) expression is significantly important in understanding the mechanism of tumor progression. In this study, we designed a special hairpin deoxyribonucleic acid (DNA) functionalized with gold nanoparticles and fluorescein isothiocyanate(FITC) as a beacon for detecting human Akt mRNA. Spectrofluorometer was used to detect the fluorescence quenching and recovery of the beacons, and laser confocal scanning microscopy was adopted to image Akt mRNA in cells. The results showed that this beacon could sensitively and quantitatively measure the Akt mRNA in living cells . This strategy is potentially useful for the cellular imaging of RNA or protein expression in living cells.

Paper Details

Date Published: 17 September 2014
PDF: 7 pages
Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 923009 (17 September 2014); doi: 10.1117/12.2068691
Show Author Affiliations
Yi Ma, China Pharmaceutical Univ. (China)
Caiping Tian, China Pharmaceutical Univ. (China)
Siwen Li, China Pharmaceutical Univ. (China)
Zhaohui Wang, China Pharmaceutical Univ. (China)
Yueqing Gu, China Pharmaceutical Univ. (China)

Published in SPIE Proceedings Vol. 9230:
Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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