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Proceedings Paper

The synchronous fluorescence spectra character of hypocrellin B with type I collagen under irradiation
Author(s): Jucheng Zhang; Heping Yan; Mengting Yang; Yongzhen Feng; Yun Huang; Lingmei Li; Wei Liu
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Paper Abstract

The synchronous fluorescence (SF) technique can provide some useful information with the endogenous fluorophores in complex systems. The SF technique has been used in the characterization of the type I collagen in PBS solution (pH=7.4) and the photo-reaction of hypocrellin B (HB) with type I collagen in solution irradiation with the 475 nm light under saturated by oxygen, air and nitrogen respectively. The SF spectra show the peaks attribute to tyrosine residues (275 nm) and pyridinoline cross-link (325 nm) in collagen. The photo-induced reaction with HB causing the fluorescence quenching but no wavelength shift, this suggests the photo-reaction don’t changing the microenvironment of PYD cross-link. The fluorescence quenching rate is faster than others when the solution saturated by oxygen. The results imply that the photo-induced reaction is oxygen dependence.

Paper Details

Date Published: 17 September 2014
PDF: 6 pages
Proc. SPIE 9230, Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014), 923005 (17 September 2014); doi: 10.1117/12.2068299
Show Author Affiliations
Jucheng Zhang, Honghe Univ. (China)
Heping Yan, Honghe Univ. (China)
Mengting Yang, Honghe Univ. (China)
Yongzhen Feng, Honghe Univ. (China)
Yun Huang, Honghe Univ. (China)
Lingmei Li, Honghe Univ. (China)
Wei Liu, Honghe Univ. (China)


Published in SPIE Proceedings Vol. 9230:
Twelfth International Conference on Photonics and Imaging in Biology and Medicine (PIBM 2014)
Qingming Luo; Lihong V. Wang; Valery V. Tuchin, Editor(s)

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