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Proceedings Paper

Spectroscopic properties of tryptophan and bacteria
Author(s): Gui Chen Tang; Yuanlong Yang; Zuo-Zhu Huang; W. Hua; F. Zhou; S. Cosloy; Robert R. Alfano
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Paper Abstract

Fluorescence spectra of tryptophan and bacteria were measured at different concentrations using a Mediscience CD-Scan unit. The emission spectra of tryptophan were obtained using an excitation wavelength at 280 nm. The excitation spectra were obtained at the emission of 340 nm. The minimum detectable concentration of tryptophan was found to be 10-8 M. The emission spectra for bacteria were probed at 340 nm. The minimum detectable number of bacteria in a beam of the excitation light was determined to be about 30. Assuming that the emission band at 340 nm of bacteria comes from tryptophan, the number of tryptophan per bacterium was estimated to be 108. This approach to determine the number is almost consistent with that obtained using a weight method.

Paper Details

Date Published: 12 April 1995
PDF: 4 pages
Proc. SPIE 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II, (12 April 1995); doi: 10.1117/12.206817
Show Author Affiliations
Gui Chen Tang, CUNY/City College (United States)
Yuanlong Yang, CUNY/City College (United States)
Zuo-Zhu Huang, CUNY/City College (United States)
W. Hua, CUNY/City College (United States)
F. Zhou, CUNY/City College (United States)
S. Cosloy, CUNY/City College (United States)
Robert R. Alfano, CUNY/City College (United States)


Published in SPIE Proceedings Vol. 2387:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II
Robert R. Alfano, Editor(s)

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