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Proceedings Paper

Surface-emitting semiconductor laser spectroscopy and microscopy for characterizing normal and sickled red blood cells
Author(s): Paul Lee Gourley; Ken E. Meissner; Thomas M. Brennan; B. Eugene Hammons; Mark F. Gourley M.D.
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Paper Abstract

We have developed a new intracavity laser technique that uses living or fixed cells at integral components of a laser. The cells are placed on an AlGaAs/GaAs surface-emitting semiconductor wafer and covered with a glass dielectric mirror to form a laser resonator. In this arrangement, the cells serve as optical waveguides (or lens elements) to confine (or focus) light generated in the resonator by the semiconductor. Because of the high transparency, the cells aid the lasing process to generate laser light. This ultra sensitive laser provides a novel imaging/spectroscopic technique for histologic examination which we demonstrate with normal and sickled human red blood cells. Extremely high contrast microscopic images of the cells are observed near 830-850 nm. These images correspond to electromagnetic modes of cell structures and are sensitive to shape of the cell. Using a high resolution spectrometer, we resolve the light emitted from these images into very narrow spectral peaks associated with the lasing modes. Analysis of the spectra reveals that the distribution of peaks is quite different for normal and sickled red blood cells. This technique, in a more developed form, may be useful for the rapid analysis of these and other kinds of normal and abnormal cells.

Paper Details

Date Published: 12 April 1995
PDF: 14 pages
Proc. SPIE 2387, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II, (12 April 1995); doi: 10.1117/12.206815
Show Author Affiliations
Paul Lee Gourley, Sandia National Labs. (United States)
Ken E. Meissner, Sandia National Labs. (United States)
Thomas M. Brennan, Sandia National Labs. (United States)
B. Eugene Hammons, Sandia National Labs. (United States)
Mark F. Gourley M.D., National Institutes of Health (United States)

Published in SPIE Proceedings Vol. 2387:
Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II
Robert R. Alfano, Editor(s)

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