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Proceedings Paper

Novel pH-sensitive probes with a ratiometric detection for intracellular pH
Author(s): Martin Ipuy; Cyrielle Billon; Guillaume Micouin; Jacques Samarut; Chantal Andraud; Yann Bretonnière
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Paper Abstract

The development of new pH-sensitive fluorescent probes based on a push-pull architecture is presented with a 2- dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofurane as strong electron acceptor group. With a small structural change, it is possible to obtain a large range of phenolic pKa from 4.8 to 8.6 with some close to neutrality, underlining the role of the electron density modulation on the acidic properties. Remarkable changes in the optical properties (both absorption and fluorescence) were observed as a function of the pH. Ratiometric imaging of intracellular pH was carried out with the most promising probes and highlighted the possibility to distinguish near-neutral minor pH fluctuations in cells.

Paper Details

Date Published: 27 August 2014
PDF: 8 pages
Proc. SPIE 9171, Nanobiosystems: Processing, Characterization, and Applications VII, 91710Q (27 August 2014); doi: 10.1117/12.2066132
Show Author Affiliations
Martin Ipuy, Lab. de Chimie, CNRS, Univ. Lyon 1, Ecole Normale Supérieure de Lyon (France)
Cyrielle Billon, Institut de Génomique Fonctionnelle de Lyon, Univ. Lyon 1, CNRS, INRA (France)
Ecole Normale Supérieure de Lyon (France)
Guillaume Micouin, Lab. de Chimie, CNRS, Univ. Lyon 1, Ecole Normale Supérieure de Lyon (France)
Jacques Samarut, Institut de Génomique Fonctionnelle de Lyon, Univ. Lyon 1, CNRS, INRA (France)
Ecole Normale Supérieure de Lyon (France)
Chantal Andraud, Lab. de Chimie, CNRS, Univ. Lyon 1, Ecole Normale Supérieure de Lyon (France)
Yann Bretonnière, Lab. de Chimie, CNRS, Univ. Lyon 1, Ecole Normale Supérieure de Lyon (France)


Published in SPIE Proceedings Vol. 9171:
Nanobiosystems: Processing, Characterization, and Applications VII
Norihisa Kobayashi; Fahima Ouchen; Ileana Rau, Editor(s)

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