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Proceedings Paper

A novel transmission electron imaging technique for observation of biological samples on a plate
Author(s): Yusuke Ominami; Masato Nakajima; Tatsuo Ushiki; Sukehiro Ito
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Paper Abstract

We introduce a novel transmission electron imaging method to clarify the internal structure of single whole cells by scanning electron microscopy (SEM). In this method, whole cells are cultivated on a transparent flat plate in advance, which is made of a scintillator that emits photons by irradiating an electron beam. The detector developed here can obtain both the secondary electron (SE) surface images and transmission electron (TE) images. Observations of whole mount cells by this technique clearly show that the cellular internal structure can be observed as transmission images which are produced by photons emitted from the scintillator plate by electron beam irradiation.

Paper Details

Date Published: 16 September 2014
PDF: 6 pages
Proc. SPIE 9236, Scanning Microscopies 2014, 923608 (16 September 2014); doi: 10.1117/12.2065479
Show Author Affiliations
Yusuke Ominami, Hitachi High-Tech Science Corp. (Japan)
Masato Nakajima, Niigata Univ. (Japan)
Tatsuo Ushiki, Niigata Univ. (Japan)
Sukehiro Ito, Hitachi High-Tech Science Corp. (Japan)

Published in SPIE Proceedings Vol. 9236:
Scanning Microscopies 2014
Michael T. Postek; Dale E. Newbury; S. Frank Platek; Tim K. Maugel, Editor(s)

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