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Proceedings Paper

Using optical lattice for STED parallelization
Author(s): Bin Yang; Frédéric Przybilla; Michael Mestre; Jean-Baptiste Trebbia; Brahim Lounis
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Paper Abstract

Being a scanning microscopy, Stimulated Emission Depletion (STED) needs to be parallelized for fast wide-field imaging. Here, we achieve large parallelization of STED microscopy using well-designed Optical Lattice (OL) for depletion, together with a fast camera for detection. Depletion optical lattices with 100 intensity “zeros” are generated by four-beam interference. Scanning only a unit cell, as small as 290 nm by 290 nm, of the depletion OL is sufficient for STED imaging. The OL-STED microscopy acquires super-resolution images with 70 nm resolution and at the speed of 80 ms per image.

Paper Details

Date Published: 28 August 2014
PDF: 6 pages
Proc. SPIE 9169, Nanoimaging and Nanospectroscopy II, 91690E (28 August 2014); doi: 10.1117/12.2064820
Show Author Affiliations
Bin Yang, Univ. Bordeaux, LP2N (France)
Institut d'Optique, CNRS, LP2N (France)
Frédéric Przybilla, Univ. Bordeaux, LP2N (France)
Institut d'Optique, CNRS, LP2N (France)
Michael Mestre, Univ. Bordeaux, LP2N (France)
Institut d'Optique, CNRS, LP2N (France)
Jean-Baptiste Trebbia, Univ. Bordeaux, LP2N (France)
Institut d'Optique, CNRS, LP2N (France)
Brahim Lounis, Univ. Bordeaux, LP2N (France)
Institut d'Optique, CNRS, LP2N (France)


Published in SPIE Proceedings Vol. 9169:
Nanoimaging and Nanospectroscopy II
Prabhat Verma; Alexander Egner, Editor(s)

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