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Proceedings Paper

Optical trapping of isolated mammalian chromosomes
Author(s): Nima Khatibzadeh; Alexander B. Stilgoe; Ann A. M. Bui; Yesenia Rocha; Gladys Cruz; Timo A. Nieminen; Halina Rubinsztein-Dunlop; Michael W. Berns
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Paper Abstract

We have estimated the mitotic forces exerted on individual isolated mammalian chromosomes using optical trapping. The chromosomes were trapped by an optical tweezers system created by a continuous wave ytterbium laser at 1064 nm. Individual chromosomes were trapped at different in situ powers in the range of ≈20-50 mW. The corresponding trapping forces were determined by a viscous drag method. In the range of laser powers used, the preliminary data show a linear relationship between the chromosome trapping forces and in situ powers. We have calculated the dimensionless trapping efficiency coefficient (Q) of the chromosomes at 1064 nm and the corresponding effects of trapping power on Q. The value of Q in our experiments was determined to be ≈0.01. The results of this study validate optical tweezers as a non-invasive and precise technique to determine intracellular forces in general, and specifically, the spindle forces exerted on the chromosomes during cell division.

Paper Details

Date Published: 16 September 2014
PDF: 7 pages
Proc. SPIE 9164, Optical Trapping and Optical Micromanipulation XI, 91642I (16 September 2014); doi: 10.1117/12.2064367
Show Author Affiliations
Nima Khatibzadeh, Univ. of California, Irvine (United States)
Alexander B. Stilgoe, The Univ. of Queensland (Australia)
Ann A. M. Bui, The Univ. of Queensland (Australia)
Yesenia Rocha, Univ. of California, Irvine (United States)
Gladys Cruz, Univ. of California, Irvine (United States)
Timo A. Nieminen, The Univ. of Queensland (Australia)
Halina Rubinsztein-Dunlop, The Univ. of Queensland (Australia)
Michael W. Berns, Univ. of California, Irvine (United States)
Univ. of California, San Diego (United States)

Published in SPIE Proceedings Vol. 9164:
Optical Trapping and Optical Micromanipulation XI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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