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Proceedings Paper

High speed fluorescence photoactivation localization microscopy imaging
Author(s): Andrew J. Nelson; Mudalige S. Gunewardene; Samuel T. Hess
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Paper Abstract

Imaging live biological samples to study biomolecular dynamics requires a very high spatial and temporal resolution. Superresolution localization microscopy has allowed researchers to investigate biological systems whose sizes are below the diffraction limit (200-250 nm) using an optical microscope. Fluorescence Photoactivation Localization Microscopy (FPALM) and other localization microscopy techniques have recently been shown to be capable of rendering superresolution images obtained with acquisitions of shorter than 0.5 seconds. Here we will discuss the FPALM imaging technique, at both lower and higher imaging speeds. This talk will focus on the advantages, challenges, and drawbacks of high speed imaging localization microscopy.

Paper Details

Date Published: 28 August 2014
PDF: 7 pages
Proc. SPIE 9169, Nanoimaging and Nanospectroscopy II, 91690P (28 August 2014); doi: 10.1117/12.2064271
Show Author Affiliations
Andrew J. Nelson, Univ. of Maine (United States)
Mudalige S. Gunewardene, Univ. of Colombo (Sri Lanka)
Samuel T. Hess, Univ. of Maine (United States)

Published in SPIE Proceedings Vol. 9169:
Nanoimaging and Nanospectroscopy II
Prabhat Verma; Alexander Egner, Editor(s)

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