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Proceedings Paper

Optoelectronic cell lysis
Author(s): Steven L. Neale; Christian Witte; Alasdair W. Clark; Julien Reboud; Jonathan M. Cooper
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Paper Abstract

In this paper we show how the electrical lysis of cells can be controlled through the use of an optoelectronic device and describe how the lysis is affected by the size and shape of the cell as well as the geometry of the device and the light patterns used. By selectively illuminating a photoconductor “virtual electrodes” are created allowing the precise control of electrical fields onto the cell within the device, electrically rupturing their membranes and allowing access to intracellular contents. We examine this optically controlled electrical lysis method and discuss its advantages as a sample preparation technique.

Paper Details

Date Published: 16 September 2014
PDF: 5 pages
Proc. SPIE 9164, Optical Trapping and Optical Micromanipulation XI, 916425 (16 September 2014); doi: 10.1117/12.2062186
Show Author Affiliations
Steven L. Neale, Univ. of Glasgow (United Kingdom)
Christian Witte, Univ. of Glasgow (United Kingdom)
Alasdair W. Clark, Univ. of Glasgow (United Kingdom)
Julien Reboud, Univ. of Glasgow (United Kingdom)
Jonathan M. Cooper, Univ. of Glasgow (United Kingdom)


Published in SPIE Proceedings Vol. 9164:
Optical Trapping and Optical Micromanipulation XI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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