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Proceedings Paper

Anomalous behavior of a three-dimensional, optically trapped, super-paramagnetic particle
Author(s): Martin Siler; Petr Jákl; Oto Brzobohatý; Jan Jezek; Pavel Zemánek
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Paper Abstract

Super-paramagnetic particles are used extensively in diagnostics and other research applications for the purification of cells, biomolecules and assays. Here, we demonstrate full 3D optical manipulation of such sub-micrometer sized particle using optical tweezers. We report three types of anomalous behavior of such a trapped particle. Firstly, the analysis of particle motion recorded by the quadrant photodiode reveals spikes in the total detected intensity that are formed by a set of very fast oscillations. Secondly, the dependence of trap stiffness on the laser power deviates from the expected linear increase for higher trapping powers. And further, such a particle cannot be optically trapped above certain trapping power threshold. We speculate that these effects are caused by particle internal structure and by its heating.

Paper Details

Date Published: 16 September 2014
PDF: 8 pages
Proc. SPIE 9164, Optical Trapping and Optical Micromanipulation XI, 916436 (16 September 2014); doi: 10.1117/12.2062148
Show Author Affiliations
Martin Siler, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Petr Jákl, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Oto Brzobohatý, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Jan Jezek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Pavel Zemánek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)


Published in SPIE Proceedings Vol. 9164:
Optical Trapping and Optical Micromanipulation XI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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