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Proceedings Paper

Force dependence of phagosome trafficking in retinal pigment epithelial cells
Author(s): Rebekah Daniel; Andrew T. Koll; David Altman
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Paper Abstract

Retinal pigment epithelial (RPE) cells play an integral role in the renewal of photoreceptor disk membranes. As rod and cone cells shed their outer segments, they are phagocytosed and degraded by the RPE, and a failure in this process can result in retinal degeneration. We have studied the role of myosin VI in nonspecific phagocytosis in a human RPE primary cell line (ARPE-19), testing the hypothesis that this motor generates the forces required to traffic phagosomes in these cells. Experiments were conducted in the presence of forces through the use of in vivo optical trapping. Our results support a role for myosin VI in phagosome trafficking and demonstrate that applied forces modulate rates of phagosome trafficking.

Paper Details

Date Published: 16 September 2014
PDF: 11 pages
Proc. SPIE 9164, Optical Trapping and Optical Micromanipulation XI, 916435 (16 September 2014); doi: 10.1117/12.2062055
Show Author Affiliations
Rebekah Daniel, Willamette Univ. (United States)
Andrew T. Koll, Willamette Univ. (United States)
David Altman, Willamette Univ. (United States)


Published in SPIE Proceedings Vol. 9164:
Optical Trapping and Optical Micromanipulation XI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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