Share Email Print
cover

Proceedings Paper

Pulsed laser activated cell sorter (PLACS) for high-throughput fluorescent mammalian cell sorting
Author(s): Yue Chen; Ting-Hsiang Wu; Aram Chung; Yu-Chung Kung; Michael A. Teitell; Dino Di Carlo; Pei-Yu Chiou
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We present a Pulsed Laser Activated Cell Sorter (PLACS) realized by exciting laser induced cavitation bubbles in a PDMS microfluidic channel to create high speed liquid jets to deflect detected fluorescent samples for high speed sorting. Pulse laser triggered cavitation bubbles can expand in few microseconds and provide a pressure higher than tens of MPa for fluid perturbation near the focused spot. This ultrafast switching mechanism has a complete on-off cycle less than 20 μsec. Two approaches have been utilized to achieve 3D sample focusing in PLACS. One is relying on multilayer PDMS channels to provide 3D hydrodynamic sheath flows. It offers accurate timing control of fast (2 m sec-1) passing particles so that synchronization with laser bubble excitation is possible, an critically important factor for high purity and high throughput sorting. PLACS with 3D hydrodynamic focusing is capable of sorting at 11,000 cells/sec with >95% purity, and 45,000 cells/sec with 45% purity using a single channel in a single step. We have also demonstrated 3D focusing using inertial flows in PLACS. This sheathless focusing approach requires 10 times lower initial cell concentration than that in sheath-based focusing and avoids severe sample dilution from high volume sheath flows. Inertia PLACS is capable of sorting at 10,000 particles sec-1 with >90% sort purity.

Paper Details

Date Published: 16 September 2014
PDF: 11 pages
Proc. SPIE 9164, Optical Trapping and Optical Micromanipulation XI, 916426 (16 September 2014); doi: 10.1117/12.2060914
Show Author Affiliations
Yue Chen, Univ. of California, Los Angeles (United States)
Ting-Hsiang Wu, Univ. of California, Los Angeles (United States)
Aram Chung, Rensselaer Polytechnic Institute (United States)
Yu-Chung Kung, Univ. of California, Los Angeles (United States)
Michael A. Teitell, Univ. of California, Los Angeles (United States)
Dino Di Carlo, Univ. of California, Los Angeles (United States)
Pei-Yu Chiou, Univ. of California, Los Angeles (United States)


Published in SPIE Proceedings Vol. 9164:
Optical Trapping and Optical Micromanipulation XI
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

© SPIE. Terms of Use
Back to Top