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Proceedings Paper

From in vitro to in vivo by dynamic multiwavelength imaging
Author(s): Daniel L. Farkas; Byron T. Ballou; Gregory W. Fisher; D. Lansing Taylor
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Paper Abstract

There is a clear trend today towards non-invasive, dynamic, digital approaches to biomedical imaging, and a need for even higher resolution. Light is particularly well suited for such investigations, as its temporal, spatial and intensity range are unparalleled. A convergence of new capabilities from fields as diverse as electronics, optics, molecular biology, computer science and dye chemistry have transformed light microscopy from a traditional, static, 2D tool into a highly useful, dynamic, 3D research capability for biology and medicine. We believe that the understanding of certain fundamental biological functions by dynamic mapping of events in living systems is within reach, based on novel, interdisciplinary methods. For imaging molecular events with high resolution (live cells, in vitro), light microscopy has continued to improve in performance, and we survey here some of our recent progress. The same dynamic mapping can be extended to organs, whole animals and humans, by monitoring molecules labeled with the long-wavelength dyes that proved useful in microscopy. We report here results obtained by in vivo imaging of fluorescently labeled monoclonal antibodies, indicative of tumor location and evolution in nude mice.

Paper Details

Date Published: 3 April 1995
PDF: 12 pages
Proc. SPIE 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, (3 April 1995); doi: 10.1117/12.206015
Show Author Affiliations
Daniel L. Farkas, Carnegie Mellon Univ. (United States)
Univ. of Pittsburgh Medical Ctr. (United States)
Byron T. Ballou, Carnegie Mellon Univ. (United States)
Univ. of Pittsburgh Medical Ctr. (United States)
Gregory W. Fisher, Carnegie Mellon Univ. (United States)
Univ. of Pittsburgh Medical Ctr. (United States)
D. Lansing Taylor, Carnegie Mellon Univ. (United States)
Univ. of Pittsburgh Medical Ctr. (United States)


Published in SPIE Proceedings Vol. 2386:
Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics
Gerald E. Cohn; Jeremy M. Lerner; Kevin J. Liddane; Alexander Scheeline; Steven A. Soper, Editor(s)

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