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Proceedings Paper

Energy transfer of nucleic acid products
Author(s): Paul M. Jung; Hsiang-Yun Hu; Omar S. Khalil
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Paper Abstract

Fluorescence energy transfer was investigated as a homogeneous detection method for the gapped ligase chain reaction (G-LCR). Oligonucleotides of a Chlamydia trachomatic G-LCR probe set were labeled with fluorescein as the donor and Texas Red as the acceptor fluorophore. Amplification and detection of 10 molecules of synthetic target was demonstrated in spiked urine samples.

Paper Details

Date Published: 3 April 1995
PDF: 10 pages
Proc. SPIE 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, (3 April 1995); doi: 10.1117/12.206013
Show Author Affiliations
Paul M. Jung, Abbott Labs. (United States)
Hsiang-Yun Hu, Abbott Labs. (United States)
Omar S. Khalil, Abbott Labs. (United States)


Published in SPIE Proceedings Vol. 2386:
Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics
Gerald E. Cohn; Jeremy M. Lerner; Kevin J. Liddane; Alexander Scheeline; Steven A. Soper, Editor(s)

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