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Proceedings Paper

Particle-enhanced total internal reflectance fluorescence®
Author(s): Victor P. Chu; Steven J. Zoha; Jim Davis; Suzette Chance
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Paper Abstract

This paper presents work toward a retrofit of an acaTMIV Discrete Clinical Analyzer (E. I. du Pont de Nemours and Company, Wilmington, DE) with a Particle Enhanced Total Internal Reflection Fluorescence technology. The DuPont acaTM line of instruments was designed to perform highly accurate unit dose clinical chemistry assays. Introduction of a Total Reflectance Fluorescence optics into an acaTMIV could provide capability to perform highly sensitive homogeneous immunoassays. Sensitivity and precision depended upon uniform immobilization of immunoglobulins or immunogens, representing examples of specific binding partners, onto an ionomeric material, acting as both solid support and wall material of the disposable reagent units called the `pack'. This study examines the uniformity of antibody coating onto various types of tough and flexible optical polymeric films including those made from the DuPont SurlynTM and NucrelTM polymers. A number of coating processes will also be described. Bioanalytical tools were developed to determine uniformity, quantity, and activity of antibody coated onto the `pack' film, thus allowing for optimization of polymer film type and process of antibody coating.

Paper Details

Date Published: 3 April 1995
PDF: 7 pages
Proc. SPIE 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, (3 April 1995); doi: 10.1117/12.206011
Show Author Affiliations
Victor P. Chu, E. I. du Pont de Nemours & Co., Inc. (United States)
Steven J. Zoha, E. I. du Pont de Nemours & Co., Inc. (United States)
Jim Davis, E. I. du Pont de Nemours & Co., Inc. (United States)
Suzette Chance, E. I. du Pont de Nemours & Co., Inc. (United States)


Published in SPIE Proceedings Vol. 2386:
Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics
Gerald E. Cohn; Jeremy M. Lerner; Kevin J. Liddane; Alexander Scheeline; Steven A. Soper, Editor(s)

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