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Proceedings Paper

Ultrahigh-speed DNA sequencing using capillary array electrophoresis chips
Author(s): Adam T. Woolley; Richard A. Mathies
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Paper Abstract

Capillary electrophoresis arrays have been fabricated on planar glass substrates using photolithographic masking and chemical etching. The photolithographically defined channel patterns were first etched in a glass substrate, and then capillaries were formed by thermally bonding the etched substrate to a second glass slide. High-speed separations of restriction fragment digests were performed on these chips in under 1 minute. Multiple separations in the same channel demonstrated excellent reproducibility. Rapid genetic typing of short tandem repeats on the HUMTHO1 locus have also been performed. Finally, separations with single- base resolution have been successful, indicating that these microfabricated devices will also be useful for high-speed DNA sequencing.

Paper Details

Date Published: 3 April 1995
PDF: 9 pages
Proc. SPIE 2386, Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics, (3 April 1995); doi: 10.1117/12.206006
Show Author Affiliations
Adam T. Woolley, Univ. of California/Berkeley (United States)
Richard A. Mathies, Univ. of California/Berkeley (United States)

Published in SPIE Proceedings Vol. 2386:
Ultrasensitive Instrumentation for DNA Sequencing and Biochemical Diagnostics
Gerald E. Cohn; Jeremy M. Lerner; Kevin J. Liddane; Alexander Scheeline; Steven A. Soper, Editor(s)

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