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Proceedings Paper

Photothermal microscopy for cell imaging and diagnostics
Author(s): Dmitry Lapotko; Georgy Kuchinsky
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Paper Abstract

Dual-laser photothermal (PT) techniques are applied for a live blood cell studies. The measurement of the action of a pump pulse (400-600 nm, 10 ns, 1-500 pJ) on a single cell provides the imaging of cell's absorbing structure/thermal field, spectroscopy and control of cell's state. The imaging technique includes acquisition of three types of images: optical image, pulsed laser image with 5 ns temporal resolution and PT-image with the same temporal and 0.5 jim spatial resolution. In PT-images of WBC the details of a cell structure have been found that can not be obtained from optical images of these cells. Quantitative analysis of PT-images enables abnormal cell differentiation in a case of anaemia (RBC) and pulinanaiy diseases (neutrophyls). Analysis of cell population according to the cell ability to survive a pump pulse is considered as the viability test. This technique was used for RBC comparative studie. Significant differences in RBC populations have been found. Also the viability test was applied for monitoiing of action of the drugs upon RBC membrane. Fully automated experimental system was built for PT-studies of the cell populations. All set up is technically compatible to optical microscope. Operation rate is 1 cell/s. The required capacity of cell suspension is about 1 j.d. Keywords: image cytometry, photothermal microscopy.

Paper Details

Date Published: 30 March 1995
PDF: 12 pages
Proc. SPIE 2390, Optical Biophysics, (30 March 1995); doi: 10.1117/12.205988
Show Author Affiliations
Dmitry Lapotko, Lykov Heat and Mass Transfer Institute (Belarus)
Georgy Kuchinsky, Lykov Heat and Mass Transfer Institute (Belarus)

Published in SPIE Proceedings Vol. 2390:
Optical Biophysics
Halina Podbielska, Editor(s)

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