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Proceedings Paper

Modulated alignment dual-axis (MAD) confocal microscopy for deep optical sectioning in tissues
Author(s): Steven Y. Leigh; Ye Chen; Jonathan T. C. Liu
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Paper Abstract

A strategy is presented to enable optical-sectioning microscopy with improved contrast and imaging depth using low-power (0.5 mW) diode laser illumination. While the DAC architecture’s intersecting illumination and collection beams significantly improves the spatial-filtering and opticalsectioning performance of confocal microscopy, we propose that modulating the spatial alignment of the dual-axis beams at a frequency f, such the focal volume signal of the microscope is modulated at 2f, further provides nearly an order-of-magnitude improvement in optical-sectioning contrast. Lock-in detection is used to remove the unmodulated background light, thereby enhancing our ability to image deeply within highly scattering tissues.

Paper Details

Date Published: 19 May 2014
PDF: 4 pages
Proc. SPIE 9155, Translational Biophotonics, 915513 (19 May 2014); doi: 10.1117/12.2057734
Show Author Affiliations
Steven Y. Leigh, Stony Brook Univ. (United States)
Ye Chen, Stony Brook Univ. (United States)
Jonathan T. C. Liu, Stony Brook Univ. (United States)

Published in SPIE Proceedings Vol. 9155:
Translational Biophotonics
Tomasz S. Tkaczyk, Editor(s)

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