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Proceedings Paper

In-line mammalian cells culture monitoring: comparison of predictive performances offered by NIR and Raman spectroscopies
Author(s): D. Didion; E. Norrant; A.-L. Hantson
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Paper Abstract

The present research proposes an experimental comparison of NIR and Raman spectroscopic techniques aiming at identifying the most efficient one for monitoring in situ and in real time suspension cultures of CHO cells. Quantitative models were developed for glucose, lactate and ammonia using the PLS algorithm. Optimization of the models was achieved by comparing several mathematical pretreatments and by performing spectral variables selection. Encouragingly LOQ’s evaluated for both techniques are smaller than limiting levels reported in literature. However the Raman instrument offers better predictive performances for each analyte most likely because of the aqueous nature of the analyzed samples.

Paper Details

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Proc. SPIE of200, OFS2013 23rd International Conference on Optical Fiber Sensors 2, ; doi: 10.1117/12.2053976
Show Author Affiliations
D. Didion, Univ. of Mons (Belgium)
E. Norrant, UCB Pharma (Belgium)
A.-L. Hantson, Univ. of Mons (Belgium)


Published in SPIE Proceedings Vol. of200:
OFS2013 23rd International Conference on Optical Fiber Sensors 2
Jesus M. Mirapeix Serrano; Manuel López-Amo; José M. López-Higuera; José M. López-Higuera; Julian D. C. Jones, Editor(s)

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