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Proceedings Paper

Multimodal Raman-fluorescence spectroscopy of formalin fixed samples is able to discriminate brain tumors from dysplastic tissue
Author(s): Suresh Anand; Riccardo Cicchi; Flavio Giordano; Anna Maria Buccoliero; Francesco Saverio Pavone
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Paper Abstract

In the recent years, there has been a considerable surge in the application of spectroscopy for disease diagnosis. Raman and fluorescence spectra provide characteristic spectral profile related to biochemical and morphological changes when tissues progress from normal state towards malignancy. Spectroscopic techniques offer the advantage of being minimally invasive compared to traditional histopathology, real time and quantitative. In biomedical optical diagnostics, freshly excised specimens are preferred for making ex-vivo spectroscopic measurements. With regard to fresh tissues, if the lab is located far away from the clinic it could pose a problem as spectral measurements have to be performed immediately after dissection. Tissue samples are usually placed in a fixative agent such as 4% formaldehyde to preserve the samples before processing them for routine histopathological studies. Fixation prevents the tissues from decomposition by arresting autolysis. In the present study, we intend to investigate the possibility of using formalin fixed samples for discrimination of brain tumours from dysplastic tissue using Raman and fluorescence spectroscopy. Formalin fixed samples were washed with phosphate buffered saline for about 5 minutes in order to remove the effects of formalin during spectroscopic measurements. In case of fluorescence spectroscopy, changes in spectral profile have been observed in the region between 550−670 nm between dysplastic and tumor samples. For Raman measurements, we found significant differences in the spectral profiles between dysplasia and tumor. In conclusion, formalin fixed samples can be potentially used for the spectroscopic discrimination of tumor against dysplastic tissue in brain samples.

Paper Details

Date Published: 8 May 2014
PDF: 5 pages
Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91293N (8 May 2014); doi: 10.1117/12.2053715
Show Author Affiliations
Suresh Anand, European Lab. for Non-Linear Spectroscopy (Italy)
Riccardo Cicchi, Istituto Nazionale di Ottica, CNR (Italy)
European Lab. for Non-Linear Spectroscopy (Italy)
Flavio Giordano, Azienda Ospedaliera Univ. Anna Meyer (Italy)
Anna Maria Buccoliero, Azienda Ospedaliera Univ. Anna Meyer (Italy)
Francesco Saverio Pavone, European Lab. for Non-Linear Spectroscopy (Italy)
Istituto Nazionale di Ottica, CNR (Italy)
Univ. degli Studi di Firenze (Italy)

Published in SPIE Proceedings Vol. 9129:
Biophotonics: Photonic Solutions for Better Health Care IV
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone; Paul Garside, Editor(s)

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