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Proceedings Paper

Simultaneous recording of t-tubular electrical activity and Ca2+-release in heart failure
Author(s): C. Crocini; R. Coppini; C. Ferrantini; P. Yan; L. Loew; C. Tesi; C. Poggesi; E. Cerbai; F. S. Pavone; L. Sacconi
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Paper Abstract

T-tubules (TT) are invaginations of the surface sarcolemma (SS) that mediate the rapid propagation of the action potential (AP) to the cardiomyocyte core. We employed the advantages of an ultrafast random access multi-photon (RAMP) microscope (Sacconi et al., PNAS 2012) with a double staining approach to optically record t-tubular AP and, simultaneously, the corresponding local Ca2+-release in different positions across the cardiomyocytes. Despite a uniform AP between SS and TT at steady-state stimulation, in control cardiomyocytes we observed a non-negligible be variability of local Ca2+-transient amplitude and kinetics. This variability was significantly reduced by applying 0.1μM Isoproterenol, which increases the opening probability of Ca2+-release units. In the rat heart failure model (HF), we previously demonstrated that some tubular elements fail to propagate AP. We found that the tubules unable to propagate AP, displayed a reduced correspondent Ca2+-transient amplitude as well as a slower Ca2+ rise compared to electrically coupled tubules. Moreover variability of Ca2+-transient kinetics were increased in HF. Finally, TT that did not show AP, occasionally exhibited spontaneous depolarizations that were never accompanied by local Ca2+-release in the absence of any pro-arrhythmogenic stimulation. Simultaneous recording of AP and Ca2+-transient allows us to probe the spatio-temporal variability of Ca2+-release, whereas the investigation of Ca2+-transient in HF discloses an unexpected uncoupling between t-tubular depolarization and Ca2+-release in remodeled tubules. This work was funded by the European Union 7th Framework Program (FP7/2007- 2013) under grant agreement n° 284464, 241526, by the Italian Ministry of University and Research (NANOMAX), and by Telethon-Italy (GGP13162).

Paper Details

Date Published: 8 May 2014
PDF: 7 pages
Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91291I (8 May 2014); doi: 10.1117/12.2053523
Show Author Affiliations
C. Crocini, European Lab. for Non-Linear Spectroscopy (Italy)
R. Coppini, Univ. degli Studi di Firenze (Italy)
C. Ferrantini, Univ. degli Studi di Firenze (Italy)
P. Yan, Univ. of Connecticut Health Ctr. (United States)
L. Loew, Univ. of Connecticut Health Ctr. (United States)
C. Tesi, Univ. degli Studi di Firenze (Italy)
C. Poggesi, Univ. degli Studi di Firenze (Italy)
E. Cerbai, Univ. degli Studi di Firenze (Italy)
F. S. Pavone, Univ. degli Studi di Firenze (Italy)
Istituto Nazionale di Ottica, CNR (Italy)
European Lab. for Non-linear Spectroscopy (Italy)
L. Sacconi, Istituto Nazionale di Ottica, CNR (Italy)
European Lab. for Non-Linear Spectroscopy (Italy)


Published in SPIE Proceedings Vol. 9129:
Biophotonics: Photonic Solutions for Better Health Care IV
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone; Paul Garside, Editor(s)

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