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Proceedings Paper

Use of stereo vision and 24-bit false color imagery to enhance visualization of multimodal confocal images
Author(s): Francesco Beltrame; Alberto Diaspro; Marco Fato; I. Martin; Paola Ramoino; Irwin E. Sobel
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Paper Abstract

Confocal microscopy systems can be linked to 3D data oriented devices for the interactive navigation of the operator through a 3D object space. Sometimes, such environments are named `virtual reality' or `augmented reality' systems. We consider optical confocal laser scanning microscopy images, in fluorescence with various excitations and emissions, and versus time The aim of our study has been the quantitative spatial analysis of confocal data using the false-color composition technique. Starting from three 2D confocal fluorescent images at the same slice location in a given biological specimen, a new single image representation of all three parameters has been generated by the false-color technique on a HP 9000/735 workstation, connected to the confocal microscope. The color composite result of the mapping of the three parameters is displayed using a resolution of 24 bits per pixel. The operator may independently vary the mix of each of the three components in the false-color composite via three (R, G, B) mixing sliders. Furthermore, by using the pixel data in the three fluorescent component images, a 3D space containing the density distribution of these three parameters has been constructed. The histogram has been displayed in stereo: it can be used for clustering purposes from the operator, through an original thresholding algorithm.

Paper Details

Date Published: 23 March 1995
PDF: 8 pages
Proc. SPIE 2412, Three-Dimensional Microscopy: Image Acquisition and Processing II, (23 March 1995); doi: 10.1117/12.205341
Show Author Affiliations
Francesco Beltrame, Univ. degli Studi di Genova (Italy)
Alberto Diaspro, Univ. degli Studi di Genova (Italy)
Marco Fato, Univ. degli Studi di Genova (Italy)
I. Martin, Univ. degli Studi di Genova (Italy)
Paola Ramoino, Univ. degli Studi di Genova (Italy)
Irwin E. Sobel, Hewlett-Packard Labs. (United States)

Published in SPIE Proceedings Vol. 2412:
Three-Dimensional Microscopy: Image Acquisition and Processing II
Tony Wilson; Carol J. Cogswell, Editor(s)

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