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Proceedings Paper

Polarized light imaging of the human brain: a new approach to the data analysis of tilted sections
Author(s): Hendrik Wiese; David Gräßel; Uwe Pietrzyk; Katrin Amunts; Markus Axer
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Paper Abstract

3D-Polarized Light Imaging (3D-PLI) is a unique technique that enables high-resolution three-dimensional mapping of the nerve fiber architecture in unstained histological sections of the human brain. 3D-PLI is based on the detection of the intrinsic tissue birefringence caused by the nerve fibers. The measured birefringent signals comprise entangled information on both spatial fiber orientation and the local fiber density. In this study, we introduce a novel approach to effectively and unambiguously unravel this interrelation, for providing a reliable estimation of fiber orientations in the entire human brain. The method relies on an in-house developed polarimetric device equipped with a tiltable specimen stage. Each brain section is measured from different perspectives and the obtained data sets are processed with a dedicated Fourier analysis optimized for fast computation and shot noise stability. For the first time it is demonstrated, that the prevailing orientations of cortical fibers can be quantified in the three-dimensional space and traced back into the white matter. Moreover, the approach provides descriptions of variances in fiber density. Hence, the method presented here opens new perspectives for the neuroanatomical study of the human cortex.

Paper Details

Date Published: 21 May 2014
PDF: 11 pages
Proc. SPIE 9099, Polarization: Measurement, Analysis, and Remote Sensing XI, 90990U (21 May 2014); doi: 10.1117/12.2053305
Show Author Affiliations
Hendrik Wiese, Forschungszentrum Jülich GmbH (Germany)
Univ. of Wuppertal (Germany)
David Gräßel, Forschungszentrum Jülich GmbH (Germany)
Uwe Pietrzyk, Forschungszentrum Jülich GmbH (Germany)
Univ. of Wuppertal (Germany)
Katrin Amunts, Forschungszentrum Jülich GmbH (Germany)
Heinrich-Heine-Univ. Düsseldorf (Germany)
Markus Axer, Forschungszentrum Jülich GmbH (Germany)

Published in SPIE Proceedings Vol. 9099:
Polarization: Measurement, Analysis, and Remote Sensing XI
David B. Chenault; Dennis H. Goldstein, Editor(s)

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