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Proceedings Paper

Pupil functions for aberration correction in 3D microscopy
Author(s): Christian K. Sieracki; Eric W. Hansen
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Paper Abstract

When a confocal fluorescence microscope with a high numerical aperture oil immersion objective is focused deep into an aqueous medium, aberrations result which weaken and blur the observed image. We have designed, fabricated and tested a two-level binary phase mask which partially corrects these aberrations, improving image brightness and resolution. A four- level mask was also designed, fabricated and tested with further brightness and resolution improvement. The mask designs and simulated and measured results are presented in this paper.

Paper Details

Date Published: 23 March 1995
PDF: 10 pages
Proc. SPIE 2412, Three-Dimensional Microscopy: Image Acquisition and Processing II, (23 March 1995); doi: 10.1117/12.205327
Show Author Affiliations
Christian K. Sieracki, Dartmouth College (United States)
Eric W. Hansen, Dartmouth College (United States)

Published in SPIE Proceedings Vol. 2412:
Three-Dimensional Microscopy: Image Acquisition and Processing II
Tony Wilson; Carol J. Cogswell, Editor(s)

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