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Proceedings Paper

Polarization contrast in scanning microscopy
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Paper Abstract

We discuss the differences between polarization contrast imaging in conventional and confocal systems and show that because of the fundamentally different imaging properties of the two systems, the extinction coefficient is non-zero in a conventional system even with perfect polars. We then discuss the axial response of high numerical aperture systems and show that a polarization effect leads to an asymmetric response when the objective lens numerical aperture is greater than the refractive index of the specimen.

Paper Details

Date Published: 23 March 1995
PDF: 6 pages
Proc. SPIE 2412, Three-Dimensional Microscopy: Image Acquisition and Processing II, (23 March 1995); doi: 10.1117/12.205323
Show Author Affiliations
Tony Wilson, Univ. of Oxford (United Kingdom)
Rimas Juskaitis, Univ. of Oxford (United Kingdom)


Published in SPIE Proceedings Vol. 2412:
Three-Dimensional Microscopy: Image Acquisition and Processing II
Tony Wilson; Carol J. Cogswell, Editor(s)

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