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Proceedings Paper

Numerical focusing in digital holographic microscopy with partially spatially coherent illumination in transmission
Author(s): Anton A. Grebenyuk; Vladimir P. Ryabukho
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Paper Abstract

Digital holographic microscopy (DHM) combines the possibility of high resolution quantitative phase imaging with numerical focusing and in transmission mode is widely used for live cell imaging. The use of partially spatially coherent illumination in DHM provides some advantages over the conventional spatially coherent illumination, but modifies the signal properties and requires detailed investigation, especially in the part of numerical focusing. In this paper we present theoretical analysis of the effects of partially spatially coherent illumination in transmission-mode DHM on its impulse response and numerical focusing possibilities. Further, we propose a method of providing high quality impulse response (as by the spatially low-coherent illumination), preserving large maximum distance of numerical focusing.

Paper Details

Date Published: 30 January 2014
PDF: 8 pages
Proc. SPIE 9031, Saratov Fall Meeting 2013: Optical Technologies in Biophysics and Medicine XV; and Laser Physics and Photonics XV, 903119 (30 January 2014); doi: 10.1117/12.2052837
Show Author Affiliations
Anton A. Grebenyuk, N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
Vladimir P. Ryabukho, N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
Institute of Precision Mechanics and Control (Russian Federation)


Published in SPIE Proceedings Vol. 9031:
Saratov Fall Meeting 2013: Optical Technologies in Biophysics and Medicine XV; and Laser Physics and Photonics XV
Elina A. Genina; Vladimir L. Derbov; Igor Meglinski; Valery V. Tuchin, Editor(s)

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